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Small Interfering RNA Targeting Nuclear Factor Kappa B to Prevent Vein Graft Stenosis in Rat Models.
- Source :
-
Transplantation Proceedings . Jul2013, Vol. 45 Issue 6, p2553-2558. 6p. - Publication Year :
- 2013
-
Abstract
- Abstract: Background: Intimal hyperplasia plays an important role in vein graft stenosis. Inflammatory injury, especially nuclear factor kappaB (NF-κB) gene activation, is highly involved in stenosis progression. We examined whether neointimal hyperplasia and vein graft stenosis could be inhibited by silencing the NF-κB gene with small interference RNA (siRNA). Methods: Sixty adult male Sprague-Dawley rats were randomly divided into a normal vein group, a vein graft group, a scrambled siRNA group, and an NF-κB siRNA group. We performed reverse interpositional grafting of the autologous external jugular vein to the abdominal aorta. Vein grafts were treated with liposome and gel complexes containing NF-κB siRNA or scrambled siRNA. The levels of monocyte chemoattractant protein -1, tumor necrosis factor-α, and NF-κB p65 in vessel tissues were evaluated after surgery for content of proliferating cell nuclear antigen (PCNA) and vascular wall thickness. Results: NF-κB siRNA treated vein graft showed less neointimal formation and fewer positive PCNA cells (P < .05). In addition there were lower levels of, NF-κB p65 protein and of inflammatory mediators (P < .05) compared with the vein graft group. Conclusion: Our study suggested that siRNA transfection suppressed NF-κB expression, reduced inflammatory factors, lessened neointimal proliferation, and suppressed PCNA. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 00411345
- Volume :
- 45
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Transplantation Proceedings
- Publication Type :
- Academic Journal
- Accession number :
- 89731234
- Full Text :
- https://doi.org/10.1016/j.transproceed.2013.03.045