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Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems.

Authors :
Mnif, Basma
Harhour, Hela
Jdidi, Jihène
Mahjoubi, Faouzia
Genel, Nathalie
Arlet, Guillaume
Hammami, Adnene
Source :
BMC Microbiology. 2013, Vol. 13 Issue 1, p1-9. 9p. 1 Diagram, 4 Charts, 1 Graph.
Publication Year :
2013

Abstract

Background: Extended-spectrum β-lactamases (ESBLs), particularly CTX-M- type ESBLs, are among the most important resistance determinants spreading worldwide in Enterobacteriaceae. The aim of this study was to characterize a collection of 163 ESBL-producing Escherichia coli collected in Tunisia, their ESBL-encoding plasmids and plasmid associated addiction systems. Results: The collection comprised 163 ESBL producers collected from two university hospitals of Sfax between 1989 and 2009. 118 isolates harbored blaCTX-M gene (101 blaCTX-M-15 gene and 17 blaCTX-M-14 gene). 49 isolates carried blaSHV-12 gene, 9 blaSHV-2a gene and only 3 blaTEM-26 gene. 16 isolates produced both CTX-M and SHV-12. The 101 CTX-M-15-producing isolates were significantly associated to phylogroup B2 and exhibiting a high number of virulence factors. 24 (23.7%) of the group B2 isolates belonged to clonal complex ST131. Pulsed-field gel electrophoresis (PFGE) typing revealed a genetic diversity of the isolates. 144 ESBL determinants were transferable mostly by conjugation. The majority of plasmid carrying blaCTX-M-15 genes (72/88) were assigned to various single replicon or multireplicon IncF types and had significantly a higher frequency of addiction systems, notably the VagCD module. Conclusion: This study demonstrates that the dissemination of CTX-M-15 producing E. coli in our setting was due to the spread of various IncF-type plasmids harboring multiple addiction systems, into related clones with high frequency of virulence determinants. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712180
Volume :
13
Issue :
1
Database :
Academic Search Index
Journal :
BMC Microbiology
Publication Type :
Academic Journal
Accession number :
89708448
Full Text :
https://doi.org/10.1186/1471-2180-13-147