The Cre/loxP recombination system for production of infectious mouse polyomavirus.

Highlights: [•] Two variants of recombinant vectors containing wild-type polyomavirus genomes flanked by loxP sites were constructed. [•] LoxP sites were introduced into the intronic region of early genes or between poly(A) signal sequences. [•] An infectious virus was obtained only from genome variant with loxP site incorporated in the intronic region. [•] Cre recombination in vivo yielded ample amount of infectious virions. [ABSTRACT FROM AUTHOR]