Interlaboratory Study of Qualitative PCR Methods for Genetically Modified Maize Events MON810, Bt11, GA21, and CaMV P35S.

The article discusses a research study on using the qualitative polymerase chain reaction (PCR) method for evaluating genetically modified (GM) maize events 35S promoter, P35SP MON810 and GA21. Participating laboratories extracted deoxyribonucleic acid (DNA) from samples and used PCR assays for detecting agrose gel electrophoresis in the products with different GM mixing samples concentration. Results showed the limit of detection (LOD) at 0.2% for MON810, 0.1% for GA21 and 0.2% for P35S.