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A General Approach to the Non-Invasive Imaging of Transgenes Using Cis-Linked Herpes Simplex Virus Thymidine Kinase.
- Source :
-
Neoplasia . Oct99, Vol. 1 Issue 4, p315. 6p. - Publication Year :
- 1999
-
Abstract
- Non-invasive imaging of gene expression opens new prospects for the study of transgenic animals and the implementation of genetically based therapies in patients. We have sought to establish a general paradigm to enable whole body non-invasive imaging of any transgene. We show that the expression and imaging of HSV1-tk (a marker gene) can be used to monitor the expression of the LacZ gene (a second gene) under the transcriptional control of a single promoter within a bicistronic unit that includes a type II internal ribosomal entry site. In cells bearing a single copy of the vector, the expression of the two genes is proportional and constant, both in vitro and in vivo. We demonstrate that non-invasive imaging of HSV1-tk gene accurately reflects the topology and activity of the other cis-linked transgene. [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENE therapy
*TRANSGENES
*DIAGNOSTIC imaging
*HERPES simplex virus
Subjects
Details
- Language :
- English
- ISSN :
- 15228002
- Volume :
- 1
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Neoplasia
- Publication Type :
- Academic Journal
- Accession number :
- 8947722
- Full Text :
- https://doi.org/10.1038/sj.neo.7900053