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Cloning, expression and purification of the SRCR domains of glycoprotein 340.

Authors :
Purushotham, Sangeetha
Deivanayagam, Champion
Source :
Protein Expression & Purification. Aug2013, Vol. 90 Issue 2, p67-73. 7p.
Publication Year :
2013

Abstract

Highlights: [•] SRCR domains of gp340 were successfully cloned with DES pMT/BiP/V5-HisA. [•] Codon optimization enhanced protein production. [•] Expressed SRCRs are folded properly and functionally adhere to S. mutans AgI/II. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
10465928
Volume :
90
Issue :
2
Database :
Academic Search Index
Journal :
Protein Expression & Purification
Publication Type :
Academic Journal
Accession number :
89343364
Full Text :
https://doi.org/10.1016/j.pep.2013.05.003