The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC–DAD–ESI-IT-TOF-MS n technique: A novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines.

Highlights: [•] A novel strategy to find absorbed constituents and metabolites of TCMs was proposed. [•] Absorption and metabolism of Paeoniae Radix Rubra were studied for the first time. [•] 13 new absorbed constituents of Paeoniae Radix Rubra were reported for the first time. [•] 90 new metabolites of PRR decoction were tentatively identified by LC–MS technique. [•] 22 new metabolites of paeoniflorin were reported for the first time. [Copyright &y& Elsevier]