Development and application of a novel, dual-mode gradient, stability-indicating HPLC-DAD method for the simultaneous determination and purity assessment of mebeverine hydrochloride, diloxanide furoate and their corresponding major degradation products in combination with some gastrointestinal drugs in the form of oral doses.

Highlights: [•] Combine a flow-rate gradient with an elution gradient known as dual-mode gradient. [•] The spectral homogeneity and purity plots and match plots. [•] Relative absorbance (RA) versus wavelengths plots. [•] Allows the fast separation of nine components within 15min cycle time. [•] DAD also supports the selectivity of the method through peak purity assessment. [Copyright &y& Elsevier]