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Liquid chromatography–tandem mass spectrometric assay for the mutated BRAF inhibitor dabrafenib in mouse plasma.
- Source :
-
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences . Apr2013, Vol. 925, p124-128. 5p. - Publication Year :
- 2013
-
Abstract
- Abstract: A quantitative bioanalytical liquid chromatography–tandem mass spectrometric (LC–MS/MS) assay for the mutated BRAF inhibitor dabrafenib was developed and validated. Plasma samples were pre-treated using protein precipitation with acetonitrile containing PLX4720 as internal standard. The extract was directly injected into the reversed-phase chromatographic system after dilution with water. The eluate was transferred into the electrospray interface with positive ionization and the analyte was detected in the selected reaction monitoring mode of a triple quadrupole mass spectrometer. The assay was validated in a 2–2000ng/ml calibration range with r 2 =0.993±0.002 for linear regression with quadratic weighting (n =5). Within day precisions (n =6) were 3.3–5.2%, between day (3 days; n =18) precisions 4.7–8.2%. Accuracies were between 95–104% for the whole calibration range. The drug was sufficiently stable under all relevant analytical conditions. Finally, the assay was successfully used to determine drug pharmacokinetics in mice. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 15700232
- Volume :
- 925
- Database :
- Academic Search Index
- Journal :
- Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 89160106
- Full Text :
- https://doi.org/10.1016/j.jchromb.2013.02.025