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High yield expression of duck hepatitis A virus VP1 protein in Escherichia coli, and production and characterization of polyclonal antibody.

Authors :
Li, Chuanfeng
Chen, Zongyan
Meng, Chunchun
Li, Lu
Liu, Guangqing
Source :
Journal of Virological Methods. Jul2013, Vol. 191 Issue 1, p69-75. 7p.
Publication Year :
2013

Abstract

Highlights: [•] Codon optimization of DHAV VP1 adapting to the codon usage bias of E. coli. [•] The expression level of the opti-VP1 gene was significantly increased in E. coli. [•] The His-tagged VP1 protein has good antigencity and immunogenicity. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
01660934
Volume :
191
Issue :
1
Database :
Academic Search Index
Journal :
Journal of Virological Methods
Publication Type :
Academic Journal
Accession number :
89103433
Full Text :
https://doi.org/10.1016/j.jviromet.2013.04.004