Back to Search
Start Over
In vitro and in vivo anti-diabetic activity of Swertia kouitchensis extract.
- Source :
-
Journal of Ethnopharmacology . Jun2013, Vol. 147 Issue 3, p622-630. 9p. - Publication Year :
- 2013
-
Abstract
- Abstract: Ethnopharmacological relevance: Swertia kouitchensis has long been used as a folk medicine to treat hepatitis and diabetes in central-western China. Therefore, this study was aimed to evaluate the anti-diabetic activity of the plant ethanol extract. Materials and methods: Firstly, the extract was tested for its inhibitory activity on α-amylase and α-glucosidase in vitro. Following that, insulin secretion test in NIT-1cell was performed. Then, oral sucrose or starch tolerance test of the extract were carried out in normal mice. After that, acute effect of the extract was executed in normal and streptozotocin-induced (60mg/kg) diabetic mice. Eventually, long term effect of the extract was performed in diabetic mice for 4 weeks. Oral glucose tolerance test and biochemical parameters were estimated at the end of the study. Results: Swertia kouitchensis extract could remarkably inhibit the activity of α-amylase and α-glucosidase and stimulate insulin secretion in vitro. And also the extract displayed anti-hyperglycemic activity, improved antioxidant capacity, ameliorated the hyperlipidemia and carbohydrate metabolism in diabetic mice. Conclusions: Swertia kouitchensis exhibits considerable anti-diabetic activity and metabolic alterations in diabetic mice. These results provide a rationale for the use of Swertia kouitchensis to treat diabetes mellitus. [Copyright &y& Elsevier]
- Subjects :
- *LIVER analysis
*MEDICINAL plants
*ALTERNATIVE medicine
*AMYLASES
*ANALYSIS of variance
*ANIMAL experimentation
*BIOLOGICAL models
*BIOPHYSICS
*DRUG toxicity
*ENZYME inhibitors
*GLUCOSE tolerance tests
*HIGH performance liquid chromatography
*HISTOLOGICAL techniques
*HYPOGLYCEMIC agents
*INSULIN
*RESEARCH methodology
*MICE
*RESEARCH funding
*STATISTICS
*PLANT extracts
*DATA analysis
*DATA analysis software
*DESCRIPTIVE statistics
*IN vitro studies
*PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 03788741
- Volume :
- 147
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Journal of Ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 89090384
- Full Text :
- https://doi.org/10.1016/j.jep.2013.03.052