An efficient in vitro refolding of recombinant bacterial laccase in Escherichia coli.

Highlights: [•] In vitro refolding of recombinant bacterial laccase from Bacillus HR03 is studied. [•] We have shown that the drop dilution as a powerful method for refolding of laccase. [•] Differences in kinetic parameters of soluble and refolded enzyme were also observed. [•] Structural changes during the refolding monitored using fluorescence spectroscopy [Copyright &y& Elsevier]