S21 - THE EFFECT OF LIGASURE USE ON ANASTOMOTIC HEALING IN COLOSTOMY.

Purpose: We aimed to investigate the effectiveness of the use of ligasure in bowel closure operation in rats. Methods: Twenty Wistar-albino rats were divided into two groups with 10 in each. In Group 1, sigmoid colon was incised from the middle and its distal was closed on it with 4/0 silk. In Group 2, sigmoid colon was divided from the middle with ligasure LS1200 probe and its distal was left in the closed form ligasure. In both of the groups, proximal sigmoid colon ans was entered in the form of colostomy with 4/0 silk to the left part of the abdomen. Tissue samples were taken for determination of tissue hydroxyproline level. The measurements of the amount of collagen tissue are determined by an amino acid, hydroxyproline. Results: While the average duration of the operation was 25,7 minutes in Group 1, it was 18,8 minutes in Group 2 and the difference between them was significant in favour of Ligasure (p<0.001). Tissue hydroxyproline level was 123,6 mg/L/gr doku (13-232) in Group 1, and 123.3 mg/L/gr doku (32-216) in Group 2 and no significant difference was determined between the groups. Adhesion level between the groups was also similar. Conclusions: This study experimentally shows that the stump can be effectively closed within a shorter time with ligasure in Hartman colostomy. LigaSure and standard methods were similar for adhesion and hydroxyproline levels. This use of Ligasure in terms of anastomotic healing shows equal success with the standard methods of colostomy. [ABSTRACT FROM AUTHOR]