S04 - PRECONCENTRATION OF TRACE ALUMINUM IN BIOLOGICAL SAMPLES WITH NEW SOLID PHASE EXTRACTION TECHNIQUE AND ITS DETERMINATION BY HIGH-RESOLUTION CONTINUUM SOURCE FLAME ATOMIC ABSORPTION SPECTROMETRY (HR-CS FAAS).

Al is a highly neurotoxic element and has been suggested to play a role in degeneration of nerve cells in the brain of human and experimental animals. It was also reported that Al was involved in the etiology of several human pathologies such as dialysis dementia, amyotrophic lateral sclerosis, senile dementia of Alzheimer type and development of the brain in fetus and suckling. Therefore, determination of aluminum in biological samples such as blood, serum, wool and urine are very important. Flame atomic absorption spectrometry (FAAS) has been widely used for the determination of trace amounts of aluminum and other metals ions. However, direct determination of metal ions in biological samples at trace levels by spectroscopic instruments as accurate and precise is limited not only due to insufficient sensitivity, but also by matrix effects. Therefore, separation and preconcentration techniques are required for determination of metal ions. In present study, a separation/preconcentration procedure for determination of aluminum in blood and urine samples has been developed by using the column containing adsorbent. Various experimental parameters (sample solution pH, sample volume, flow rate of sample solution, volume and concentration of eluent) and analytical parameters (Lineer range, limit of detection, preconcentration factor, accurate and precise) were investigated. Aluminum ions in samples were complexed with 4-[(dicyanomethyl)diazenyl] benzoic acid (DSMDABA) and, were separated/preconcentrated on polymeric resin (Duolite XAD-761) in column. The adsorbed aluminum on resin was eluted with 2 mol L-1 HNO3 and its concentration was determined HR-CS FAAS. [ABSTRACT FROM AUTHOR]