Back to Search
Start Over
Inhibition of Lipopolysaccharide-Induced iNOS and COX-2 Expression by Indole Alkaloid, 3-(Hydroxymethyl)-6,7-Dihydroindolo [2,3-a]Quinolizin-(12H)-one, via NF-κB Inactivation in RAW 264.7 Macrophages.
- Source :
-
Planta Medica . Jun2013, Vol. 79 Issue 9, p782-787. 6p. - Publication Year :
- 2013
-
Abstract
- 3-(Hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one is a bioactive indole alkaloid isolated from Nauclea officinalis, a plant species which is used as a traditional Chinese medicine. We investigated the anti-inflammatory properties of 3-(hydroxymethyl)-6,7-dihydroindolo [2,3-a]quinolizin-(12H)-one in RAW 264.7 murinemacrophages. The results indicated that it inhibited the overproduction of NO and the release of TNF-α. Furthermore, this compound inhibited the expression of iNOS and COX-2 proteins, the enzymatic activity of iNOS, and the translocation of NF-κB to the nucleus induced by LPS. Therefore, we suggested that the effect of 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one-mediated inhibition of the expression of LPS-induced iNOS and COX-2 genes is due to the suppression of NF-κB activation in the transcriptional level. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ALKALOIDS
*ANALYSIS of variance
*ANIMAL experimentation
*BIOLOGICAL assay
*CELL culture
*ENZYME-linked immunosorbent assay
*GENE expression
*MICE
*MOLECULAR structure
*NITRIC oxide
*NUCLEAR magnetic resonance spectroscopy
*OXIDOREDUCTASES
*RESEARCH funding
*STATISTICS
*WESTERN immunoblotting
*PLANT extracts
*DATA analysis
*DATA analysis software
*LIPOPOLYSACCHARIDES
*DESCRIPTIVE statistics
*CHEMICAL inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 00320943
- Volume :
- 79
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Planta Medica
- Publication Type :
- Periodical
- Accession number :
- 88361132
- Full Text :
- https://doi.org/10.1055/s-0032-1328550