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Monomeric TonB and the Ton Box Are Required for the Formation of a High-Affinity Transporter--TonB Complex.

Authors :
Freed, Daniel M.
Lukasik, Stephen M.
Sikora, Arthur
Mokdad, Audrey
Cafiso, David S.
Source :
Biochemistry. 4/16/2013, Vol. 52 Issue 15, p2638-2648. 11p.
Publication Year :
2013

Abstract

The article presents a study which characterizes the interaction between TonB and the transporters BtuB, FhuA, and FecA. A combination of fluoresence anisotropy and electromagnetic paramagnetic resonance spectroscopy was used in the study. The construction of the TonB of binds with high affinity to the transporter, which increases the presence of substrate, is discussed.

Details

Language :
English
ISSN :
00062960
Volume :
52
Issue :
15
Database :
Academic Search Index
Journal :
Biochemistry
Publication Type :
Academic Journal
Accession number :
88311959
Full Text :
https://doi.org/10.1021/bi3016108