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Adenovirus-mediated in vivo B7-1 gene transfer induces anti–tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma.
- Source :
-
Cancer Gene Therapy . Sep2002, Vol. 9 Issue 9, p747. 9p. - Publication Year :
- 2002
-
Abstract
- We have previously reported that an osteosarcoma vaccine generated by ex vivo transfection of B7-1 cDNA induces protective as well as curative immunity against B7-1–negative parental osteosarcoma. Because establishment of human osteosarcoma cell lines, which is a prerequisite for ex vivo gene transfer, is rarely successful, we, in the present study, investigated the therapeutic efficacy of adenovirus-mediated in vivo B7-1 gene transfer to pre-established primary tumor as well as pulmonary metastasis of osteosarcoma. Adenovirus-mediated rat B7-1 gene transfer induced (a) expression of B7-1 molecules in osteosarcoma cells by both in vitro and in vivo infection procedures, (b) curative immunity against pre-established primary osteosarcoma and, subsequently, hosts gained protection against additional challenge of parental B7-1–negative osteosarcoma cells, (c) systemic immunity against pre-established pulmonary metastasis, and (d) activation of regional lymph node CD4+ T cells, expansion of dendritic cells and natural killer cells and the secretion of interferon-γ. These findings collectively support the therapeutic value of adenovirus-mediated in vivo gene transfer on osteosarcoma, which is of greater simplicity than cell-based B7-1 vaccine, and represent an attractive strategy for therapy of patients with metastatic osteosarcama who acquired resistance to current therapeutic protocols. Cancer Gene Therapy (2002) 9, 747–755 doi:10.1038/sj.cgt.7700491 [ABSTRACT FROM AUTHOR]
- Subjects :
- *GENETIC transformation
*OSTEOSARCOMA
*ADENOVIRUSES
*IMMUNOTHERAPY
Subjects
Details
- Language :
- English
- ISSN :
- 09291903
- Volume :
- 9
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Cancer Gene Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 8822783
- Full Text :
- https://doi.org/10.1038/sj.cgt.7700491