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scAAV-mediated gene transfer of interleukin-1-receptor antagonist to synovium and articular cartilage in large mammalian joints.
- Source :
-
Gene Therapy . Jun2013, Vol. 20 Issue 6, p670-677. 8p. 1 Color Photograph, 2 Diagrams, 3 Graphs. - Publication Year :
- 2013
-
Abstract
- With the long-term goal of developing a gene-based treatment for osteoarthritis (OA), we performed studies to evaluate the equine joint as a model for adeno-associated virus (AAV)-mediated gene transfer to large, weight-bearing human joints. A self-complementary AAV2 vector containing the coding regions for human interleukin-1-receptor antagonist (hIL-1Ra) or green fluorescent protein was packaged in AAV capsid serotypes 1, 2, 5, 8 and 9. Following infection of human and equine synovial fibroblasts in culture, we found that both were only receptive to transduction with AAV1, 2 and 5. For these serotypes, however, transgene expression from the equine cells was consistently at least 10-fold higher. Analyses of AAV surface receptor molecules and intracellular trafficking of vector genomes implicate enhanced viral uptake by the equine cells. Following delivery of 1 × 1011 vector genomes of serotypes 2, 5 and 8 into the forelimb joints of the horse, all three enabled hIL-1Ra expression at biologically relevant levels and effectively transduced the same cell types, primarily synovial fibroblasts and, to a lesser degree, chondrocytes in articular cartilage. These results provide optimism that AAV vectors can be effectively adapted for gene delivery to large human joints affected by OA. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09697128
- Volume :
- 20
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Gene Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 87970067
- Full Text :
- https://doi.org/10.1038/gt.2012.81