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A new family of plant transcription factors displays a novel ssDNA-binding surface.
- Source :
-
Nature Structural Biology . Jul2002, Vol. 9 Issue 7, p512. 6p. 3 Diagrams, 1 Chart, 1 Graph. - Publication Year :
- 2002
-
Abstract
- The crystal structure of p24, the single-stranded DNA (ssDNA) binding subunit of the plant defense transcription factor PBF-2, has been determined to 2.3 Å resolution. p24 is representative of a novel family of ubiquitous plant-specific proteins that we refer to as the Whirly family because of their quaternary structure. PBF-2 is composed of four p24 molecules that interact through a helix-loop-helix motif. This interaction produces a central pore, with β-strands radiating outwards, resulting in a whirligig appearance to the quaternary structure. The noncrystallographic C4 symmetry arrangement of p24 subunits is novel for ssDNA binding proteins and may explain the binding specificity of PBF-2. This structural arrangement also supports the role of PBF-2 in binding melted promoter regions to modulate gene expression. [ABSTRACT FROM AUTHOR]
- Subjects :
- *TRANSCRIPTION factors
*PLANT genetics
*BINDING sites
Subjects
Details
- Language :
- English
- ISSN :
- 10728368
- Volume :
- 9
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Nature Structural Biology
- Publication Type :
- Academic Journal
- Accession number :
- 8782018
- Full Text :
- https://doi.org/10.1038/nsb814