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SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.

Authors :
Zhao, Xiao-Hui
Han, Fa
Li, Yu-Lin
Zhou, Guo-Ying
Yue, Hui-Lan
Source :
Journal of Liquid Chromatography & Related Technologies. Jun2013, Vol. 36 Issue 13, p1751-1761. 11p. 1 Diagram, 2 Charts, 2 Graphs.
Publication Year :
2013

Abstract

An effective high–speed counter–current chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plantPedicularis longifloravar.tubiformis(Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroform–methanol–water (8:4:5, v/v), 40 mg of extract was separated to yield luteolin (12.5 mg), apigenin (9.6 mg), and chrysoeriol (4.8 mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by1H NMR and13C NMR. [ABSTRACT FROM PUBLISHER]

Details

Language :
English
ISSN :
10826076
Volume :
36
Issue :
13
Database :
Academic Search Index
Journal :
Journal of Liquid Chromatography & Related Technologies
Publication Type :
Academic Journal
Accession number :
87568182
Full Text :
https://doi.org/10.1080/10826076.2012.698677