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SEMI-PREPARATIVE SEPARATION AND PURIFICATION OF THREE FLAVONOIDS FROM PEDICULARIS LONGIFLORA VAR. TUBIFORMIS (KLOTZSCH) P. C. TSOONG BY HSCCC.
- Source :
-
Journal of Liquid Chromatography & Related Technologies . Jun2013, Vol. 36 Issue 13, p1751-1761. 11p. 1 Diagram, 2 Charts, 2 Graphs. - Publication Year :
- 2013
-
Abstract
- An effective high–speed counter–current chromatography (HSCCC) method was established for semi-preparative isolation and purification of bioactive chemical constituents from the Tibetan medicinal plantPedicularis longifloravar.tubiformis(Klotzsch) P. C. Tsoong. With a two-phase solvent system composed of chloroform–methanol–water (8:4:5, v/v), 40 mg of extract was separated to yield luteolin (12.5 mg), apigenin (9.6 mg), and chrysoeriol (4.8 mg), with purities of 99.3, 98.2, and 98.6%, respectively, as determined by high-performance liquid chromatography (HPLC). The chemical structures of these three components were identified by1H NMR and13C NMR. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 10826076
- Volume :
- 36
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Journal of Liquid Chromatography & Related Technologies
- Publication Type :
- Academic Journal
- Accession number :
- 87568182
- Full Text :
- https://doi.org/10.1080/10826076.2012.698677