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BINDING OF A PORPHYRIN CONJUGATE OF HOECHST 33258 TO DNA. II. NMR SPECTROSCOPIC STUDIES DETECT MULTIPLE BINDING MODES TO A 12-MER NONSELF-COMPLEMENTARY DUPLEX DNA.
- Source :
-
Nucleosides, Nucleotides & Nucleic Acids . Jan2001, Vol. 20 Issue 1/2, p145. 12p. - Publication Year :
- 2001
-
Abstract
- We have probed by 1H NMR spectroscopy the molecular basis of the interaction between Hoechst 33258 conjugated to a des-metalloporphyrin and a non self-complementary duplex DNA sequence, designed on the known chemical nuclease selectivity of this system. The imino NMR spectra are consistent with two distinct families of structure, that is, PORHOE binding either way along the duplex. 2D spectral, T2, and linewidth data suggest multiple species within the two conformational families. [ABSTRACT FROM AUTHOR]
- Subjects :
- *NUCLEAR magnetic resonance spectroscopy
*NUCLEOTIDE sequence
*NUCLEASES
Subjects
Details
- Language :
- English
- ISSN :
- 15257770
- Volume :
- 20
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Nucleosides, Nucleotides & Nucleic Acids
- Publication Type :
- Academic Journal
- Accession number :
- 8739440
- Full Text :
- https://doi.org/10.1081/NCN-100001443