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Fabrication of a novel porous PGA-chitosan hybrid matrix for tissue engineering

Authors :
Wang, Yu-Chi
Lin, Mei-Chiao
Wang, Da-Ming
Hsieh, Hsyue-Jen
Source :
Biomaterials. Mar2003, Vol. 24 Issue 6, p1047. 11p.
Publication Year :
2003

Abstract

Polyglycolide (PGA) and chitosan mixture solution was prepared using solvents of low toxicity to create novel, porous, biocompatible, degradable, and modifiable hybrid matrices for biomedical applications. The porosity of these PGA-chitosan hybrid matrices (P/C matrices) was created by a thermally induced phase separation method. Two types of the P/C hybrid matrices containing 70 wt% PGA (P/C-1 matrix) and 30 wt% PGA (P/C-2 matrix) were fabricated. Chitosan matrix was also prepared for comparison. A 35-day in vitro degradation revealed that the weight losses for the P/C-1 and P/C-2 matrices were similar (∼61%), but the releases of glycolic acid from the P/C-1 and P/C-2 matrices were 95% and 60%, respectively. The P/C-1 matrix had higher porosity and higher mechanical strength than the P/C-2 and chitosan matrices. Fibroblast cells cultivated in these matrices proliferated well and the cell density was the highest in the P/C-1 matrix, followed by the chitosan and P/C-2 matrices, suggesting good biocompatibility for the P/C-1 matrix. We thereby concluded that the P/C-1 matrix, due to its high strength, porosity, biocompatibility and degradability, is a promising biomaterial. The presence of chitosan in the P/C matrices provides many amino groups for further modifications such as biomolecule conjugation and thus enhances the application potential of the P/C hybrid matrices in tissue engineering. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
01429612
Volume :
24
Issue :
6
Database :
Academic Search Index
Journal :
Biomaterials
Publication Type :
Academic Journal
Accession number :
8722760
Full Text :
https://doi.org/10.1016/S0142-9612(02)00434-9