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Flow cytometry thresholds of myeloperoxidase detection to discriminate between acute lymphoblastic or myeloblastic leukaemia.
- Source :
-
British Journal of Haematology . May2013, Vol. 161 Issue 4, p551-555. 5p. 2 Graphs. - Publication Year :
- 2013
-
Abstract
- The World Health Organization 2008 Classification emphasizes myeloperoxidase ( MPO) detection as sufficient for assigning a blast population to the myeloid lineage. Published MPO positivity thresholds are 10% for flow cytometry ( FCM) but 3% for cytochemistry. Here we re-evaluated the FCM- MPO threshold by comparing retrospectively 128 acute lymphoblastic leukaemias and 75 acute myeloid leukaemias without maturation, all assessed by benzidine-based cytochemistry. A 13% threshold was found to be relevant using an isotype control as background-reference (sensitivity 95·1%, specificity 91·7%). Residual normal lymphocytes proved to be an advantageous alternative reference, a threshold of 28% yielding improved 97·4% sensitivity and 96·1% specificity. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00071048
- Volume :
- 161
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- British Journal of Haematology
- Publication Type :
- Academic Journal
- Accession number :
- 87106225
- Full Text :
- https://doi.org/10.1111/bjh.12277