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Rapid Detection of Norovirus from Fresh Lettuce Using Immunomagnetic Separation and a Quantum Dots Assay.
- Source :
-
Journal of Food Protection . Apr2013, Vol. 76 Issue 4, p707-711. 5p. - Publication Year :
- 2013
-
Abstract
- Current molecular methods that include PCR have been used to detect norovirus in many food samples. However, the protocols require removing PCR inhibitors and incorporate time-consuming concentration steps to separate virus from analyte for rapid and sensitive detection of norovirus. We developed an immunomagnetic separation (IMS) and a quantum dots (QDs) assay to detect norovirus eluted. from fresh lettuce with Tris buffer containing 1 % beef extract (pH 9.5). IMS facilitated viral precipitation with a 10-min incubation, whereas virus concentration using polyethylene glycol (PEG) requires more than 3 h and an additional high-speed centrifugation step to precipitate virus before reverse transcription PCR (RT-PCR) analysis. The fluorescence intensity of QDs was detected qualitatively on norovirus dilutions of 10-1 to 10-3 in a stool suspension (100 RT-PCR units/ml). The results suggest that a fluorescence assay based on IMS and QDs is valid for detecting norovirus qualitatively according to fluorescent signal intensity within the same virus detection limit produced by IMS-RT-PCR and PEG-RT-PCR. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0362028X
- Volume :
- 76
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Journal of Food Protection
- Publication Type :
- Academic Journal
- Accession number :
- 87049325
- Full Text :
- https://doi.org/10.4315/0362-028X.JFP-12-343