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Comparison of the Neural Differentiation Potential of Human Mesenchymal Stem Cells from Amniotic Fluid and Adult Bone Marrow.
- Source :
-
Cellular & Molecular Neurobiology . May2013, Vol. 33 Issue 4, p465-475. 11p. - Publication Year :
- 2013
-
Abstract
- Human mesenchymal stem cells (MSCs) are considered a promising tool for cell-based therapies of nervous system diseases. Bone marrow (BM) has been the traditional source of MSCs (BM-MSCs). However, there are some limitations for their clinical use, such as the decline in cell number and differentiation potential with age. Recently, amniotic fluid (AF)-derived MSCs (AF-MSCs) have been shown to express embryonic and adult stem cell markers, and can differentiate into cells of all three germ layers. In this study, we isolated AF-MSCs from second-trimester AF by limiting dilution and compared their proliferative capacity, multipotency, neural differentiation ability, and secretion of neurotrophins to those of BM-MSCs. AF-MSCs showed a higher proliferative capacity and more rapidly formed and expanded neurospheres compared to those of BM-MSCs. Both immunocytochemical and quantitative real-time PCR analyses demonstrated that AF-MSCs showed higher expression of neural stemness markers than those of BM-MSCs following neural stem cell (NSC) differentiation. Furthermore, the levels of brain-derived growth factor and nerve growth factor secreted by AF-MSCs in the culture medium were higher than those of BM-MSCs. In addition, AF-MSCs maintained a normal karyotype in long-term cultures after NSC differentiation and were not tumorigenic in vivo. Our findings suggest that AF-MSCs are a promising and safe alternative to BM-MSCs for therapy of nervous system diseases. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MESENCHYMAL stem cells
*AMNIOTIC liquid
*BONE marrow
*NEURONS
*BIOMARKERS
Subjects
Details
- Language :
- English
- ISSN :
- 02724340
- Volume :
- 33
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Cellular & Molecular Neurobiology
- Publication Type :
- Academic Journal
- Accession number :
- 86727365
- Full Text :
- https://doi.org/10.1007/s10571-013-9922-y