Back to Search
Start Over
Effect of amiloride on ischaemia and reperfusion injury in isolated, perfused rat hearts.
- Source :
-
Scandinavian Cardiovascular Journal . May98, Vol. 32 Issue 3, p167-172. 6p. - Publication Year :
- 1998
-
Abstract
- The effect of amiloride, a potent inhibitor of Na+/H+ exchange, on ischaemic reperfused rat hearts was studied in order to investigate whether Na+/H+ exchange or Na+/Ca2+ exchange is involved in ischaemia-reperfusion injury, When hearts were pre-ischaemically loaded with 100 microM amiloride, recovery of left ventricular developed pressure was significantly better than in control hearts, whereas recovery of heart rate at 30-min reperfusion was unaffected. Amiloride pretreatment also decreased creatine phosphokinase activity in the coronary effluent and completely abolished occurrence of ventricular arrhythmias during reperfusion. It also inhibited intracellular Na+ accumulation early in reperfusion (within 5 min), whereas in the late stage (from 5 to 30 min), Ca2+ overload was inhibited. The findings suggest that Na+/H+ exchange participates mainly in the early stage of reperfusion injury and the Na+/Ca2+ exchange system, secondary to Na+/H+ exchange, in the late stage. The reduction in post-ischaemic cardiac dysfunction induced by amiloride pretreatment may be attributable to inhibition of the resultant Ca2+ accumulation during reperfusion. [ABSTRACT FROM AUTHOR]
- Subjects :
- *AMILORIDE
*REPERFUSION injury
*ISCHEMIA
*LABORATORY mice
Subjects
Details
- Language :
- English
- ISSN :
- 14017431
- Volume :
- 32
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Scandinavian Cardiovascular Journal
- Publication Type :
- Academic Journal
- Accession number :
- 8664928
- Full Text :
- https://doi.org/10.1080/14017439850140148