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Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows.
- Source :
-
Journal of Veterinary Pharmacology & Therapeutics . Apr2013, Vol. 36 Issue 2, p130-139. 10p. 2 Charts, 8 Graphs. - Publication Year :
- 2013
-
Abstract
- Donalisio, C., Barbero, R., Cuniberti, B., Vercelli, C., Casalone, M., Re, G. Effects of flunixin meglumine and ketoprofen on mediator production in ex vivo and in vitro models of inflammation in healthy dairy cows. J. vet. Pharmacol. Therap. 36, 130-139. In this study, ex vivo assays were carried out in dairy cows to evaluate the anti-inflammatory effects of two nonsteroidal anti-inflammatory drugs: ketoprofen (KETO) and flunixin meglumine (FM). Twelve healthy Holstein dairy cattle were randomly allocated to two groups ( n=6): group 1 received FM and group 2 received KETO at recommended therapeutic dosages. The anti-inflammatory effects of both drugs were determined by measuring the production of coagulation-induced thromboxane B2 (TXB2), lipopolysaccharides (LPS) (10 μg/mL)-induced prostaglandin E2 (PGE2), and calcium ionophore (60 μ m)-induced leukotrien B4 (LTB4). Cytokine production was assessed by measuring tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) and interleukin-8 (CXCL8) concentrations after incubation in the presence of 10 μg/mL LPS. The IC50 of FM and KETO was determined in vitro by determining the concentration of TXB2 and PGE2 in the presence of scalar drug concentrations (10−9-10−3 m). Both FM and KETO inhibited the two COX isoforms in vitro, but showed a preference for COX-1. FM and KETO showed similar anti-inflammatory effects in the cow. [ABSTRACT FROM AUTHOR]
- Subjects :
- *DAIRY cattle
*INFLAMMATION
*CYTOKINES
*DRUGS
*ANTIVIRAL agents
Subjects
Details
- Language :
- English
- ISSN :
- 01407783
- Volume :
- 36
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Journal of Veterinary Pharmacology & Therapeutics
- Publication Type :
- Academic Journal
- Accession number :
- 85974718
- Full Text :
- https://doi.org/10.1111/j.1365-2885.2012.01396.x