Effect of gamma radiation on the identification of bacterial pathogens by MALDI-TOF MS

Authors :: Tracz, Dobryan M.
McCorrister, Stuart J.
Westmacott, Garrett R.
Corbett, Cindi R.
Source :: Journal of Microbiological Methods. Feb2013, Vol. 92 Issue 2, p132-134. 3p.
Publication Year :: 2013
Abstract: Abstract: MALDI-TOF MS is a well-established method for rapid identification of bacteria; however there are no reports to date on its performance with gamma-irradiated samples typically used in BSL-3 laboratories for sample inactivation. In this report we demonstrate that gamma-irradiated bacteria can be accurately identified by MALDI-TOF MS in most cases, but a decrease in identification scores is observed. [Copyright &y& Elsevier]

Subjects :: *GAMMA rays
*ENZYME-linked immunosorbent assay
*BIOSAFETY
*MASS spectrometry
*MATRIX-assisted laser desorption-ionization
*TIME-of-flight mass spectrometry
*PATHOGENIC bacteria
*RAPID methods (Microbiology)

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