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EFFECTS OF POLY(ETHYLENEGLYCOL)-MODIFIED HEMOGLOBIN VESICLES ON N-FORMYL-METHIONYL-LEUCYL-PHENYLALANINE-INDUCED RESPONSES OF POLYMORPHONUCLEAR NEUTROPHILS IN VITRO.
- Source :
-
Artificial Cells, Blood Substitutes, & Immobilization Biotechnology . Nov2001, Vol. 29 Issue 6, p427. 11p. - Publication Year :
- 2001
-
Abstract
- [Poly(ethyleneglycol)]-modified hemoglobin vesicles (PEG-HbV), a type of encapsulated hemoglobin, have been developed as artificial oxygen carriers and it is important to evaluate their blood compatibility. We studied the effects of PEG-HbV on human polymorphonuclear neutrophils (PMNs) in vitro, focusing on the functional responses to N-formyl-methionyl-leucyl-phenylalanine (fMLP) as an agonist. The pretreatment of the PMNs with PEG-HbV up to a concentration of 60 mg/dl Hb did not affect the fMLP-triggered chemotactic activity. In parallel to these results, the fMLP-induced upregulation of CD11b(Mac-1) levels on the PEG-HbV-pretreated PMNs was comparable to that of untreated cells. Furthermore, the pretreatment of the PMNs with the PEG-HbV even at 600 mg/dl Hb did not affect the gelatinase B (Matrix methalloproteinase-9 (MMP-9)) release, suggesting that the fMLP-induced release of secondary and tertiary granules was normal. In addition, the fMLP-triggered superoxide production of the PMNs was unchanged by the pretreatment with the PEG-HbV at 600 mg/dl Hb. Thus, these results suggest that PEG-HbV, at the concentrations studied, have no aberrant effects on the fMLP-triggered functions of human PMNs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HEMOGLOBINS
*NEUTROPHILS
*PHENYLALANINE
Subjects
Details
- Language :
- English
- ISSN :
- 10731199
- Volume :
- 29
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Artificial Cells, Blood Substitutes, & Immobilization Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 8535584
- Full Text :
- https://doi.org/10.1081/BIO-100108548