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The Nucleotide Sequence of RNA1 of Lettuce big-vein virus, Genus Varicosavirus, Reveals Its Relation to Nonsegmented Negative-Strand RNA Viruses
- Source :
-
Virology . Jun2002, Vol. 297 Issue 2, p289. 9p. - Publication Year :
- 2002
-
Abstract
- The complete nucleotide sequence of RNA1 from Lettuce big-vein virus (LBVV), the type member of the genus Varicosavirus, was determined. LBVV RNA1 consists of 6797 nucleotides and contains one large ORF that encodes a large (L) protein of 2040 amino acids with a predicted Mr of 232,092. Northern blot hybridization analysis indicated that the LBVV RNA1 is a negative-sense RNA. Database searches showed that the amino acid sequence of L protein is homologous to those of L polymerases of nonsegmented negative-strand RNA viruses. A cluster dendrogram derived from alignments of the LBVV L protein and the L polymerases indicated that the L protein is most closely related to the L polymerases of plant rhabdoviruses. Transcription termination/polyadenylation signal-like poly(U) tracts that resemble those in rhabdovirus and paramyxovirus RNAs were present upstream and downstream of the coding region. Although LBVV is related to rhabdoviruses, a key distinguishing feature is that the genome of LBVV is segmented. The results reemphasize the need to reconsider the taxonomic position of varicosaviruses. [Copyright &y& Elsevier]
- Subjects :
- *VEIN diseases
*NUCLEOTIDE sequence
*RNA viruses
*NEGATIVE-strand RNA viruses
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Volume :
- 297
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Virology
- Publication Type :
- Academic Journal
- Accession number :
- 8506709
- Full Text :
- https://doi.org/10.1006/viro.2002.1420