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Retroviral Transduction and Engraftment Ability of Primate Hematopoietic Progenitor and Stem Cells Transduced Under Serum-Free versus Serum-Containing Conditions
- Source :
-
Molecular Therapy . Mar2002, Vol. 5 Issue 3, p316. 7p. - Publication Year :
- 2002
-
Abstract
- The ability to efficiently transduce hematopoietic stem and progenitor cells under serum-free conditions would be desirable for safety and standardization of clinical gene therapy protocols. Using rhesus macaques, we studied the transduction efficiency and engraftment ability of CD34-enriched SCF/G-CSF mobilized progenitor cells (PBSC) transduced with standard amphotropic marking vectors under serum-free and serum-containing conditions. Supernatants were collected from producer cells 16 hours after serum-free medium or medium containing 10% fetal calf serum was added. Vector titers were approximately two- to threefold higher when producer cells were cultured in serum-containing medium. However, retroviral transduction of rhesus CFU-GM was improved using serum-free vector-containing medium. For analysis of engraftment with transduced cells, three macaques had CD34+ peripheral blood stem cells split into two fractions for transduction. One fraction was transduced using serum-free vector-containing medium, and the other fraction was transduced using standard serum-containing medium. The two fractions were re-infused simultaneously following total body irradiation. In all three animals, there was equivalent marking from both vectors for 7–9 months post-transplantation. These data are encouraging regarding the removal of serum-containing medium from clinical hematopoietic cell transduction protocols, given the lack of a detrimental effect on transduction and engraftment with transduced cells. [Copyright &y& Elsevier]
- Subjects :
- *GENETIC transduction
*RETROVIRUS genetics
*STEM cells
Subjects
Details
- Language :
- English
- ISSN :
- 15250016
- Volume :
- 5
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Molecular Therapy
- Publication Type :
- Academic Journal
- Accession number :
- 8504328
- Full Text :
- https://doi.org/10.1006/mthe.2002.0544