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Quantitative Assessment of the Interplay Between DNA Elasticity and Cooperative Binding of Ligands.

Authors :
Siman, L.
Carrasco, I. S. S.
Silva, J. K. L. da
de Oliveira, M. C.
Rocha, M. S.
Mesquita, O. N.
Source :
Physical Review Letters. 12/14/2012, Vol. 109 Issue 24, p248103-1-248103-5. 5p.
Publication Year :
2012

Abstract

Binding of ligands to DNA can be studied by measuring the change of the persistence length of the complex formed, in single-molecule assays. We propose a methodology for persistence length data analysis based on a quenched disorder statistical model and describing the binding isotherm by a Hill-type equation. We obtain an expression for the effective persistence length as a function of the total ligand concentration, which we apply to our data of the DNA-cationic β-cyclodextrin and to the DNA-HU protein data available in the literature, determining the values of the local persistence lengths, the dissociation constant, and the degree of cooperativity for each set of data. In both cases the persistence length behaves nonmonotonically as a function of ligand concentration and based on the results obtained we discuss some physical aspects of the interplay between DNA elasticity and cooperative binding of ligands. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00319007
Volume :
109
Issue :
24
Database :
Academic Search Index
Journal :
Physical Review Letters
Publication Type :
Academic Journal
Accession number :
84924442
Full Text :
https://doi.org/10.1103/PhysRevLett.109.248103