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Proteomic identification of camel seminal plasma: Purification of β-nerve growth factor

Authors :
Kumar, Sanjay
Sharma, Vinay Kumar
Singh, Sudhuman
Hariprasad, Guru Rao
Mal, Gorakh
Srinivasan, Alagiri
Yadav, Savita
Source :
Animal Reproduction Science. Jan2013, Vol. 136 Issue 4, p289-295. 7p.
Publication Year :
2013

Abstract

Abstract: The camel seminal plasma contains a diverse array of components including lipids, carbohydrates, peptides, ions and proteins. These are essential for maintaining normal physiology of spermatozoa and are secreted mainly from the prostrate, epidydimis and bulbo-urethral glands of reproductive system. The protein profiles of camel seminal plasma were resolved by two-dimensional gel electrophoresis (2D-PAGE). The majority of the protein was found in acidic regions below pI 7.0 and the 19 brightly stained proteins were identified by MALDI-TOF/MS analysis. On the basis of proteomic profiles, β-nerve growth factor (β-NGF) was purified by ion-exchange and gel filtration chromatography and identified by SDS-PAGE and MALDI-TOF/MS analysis. It was further confirmed by western blotting experiments using rabbit anti-β-NGF primary antibody. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
03784320
Volume :
136
Issue :
4
Database :
Academic Search Index
Journal :
Animal Reproduction Science
Publication Type :
Academic Journal
Accession number :
84574143
Full Text :
https://doi.org/10.1016/j.anireprosci.2012.11.001