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Proteomic identification of camel seminal plasma: Purification of β-nerve growth factor
- Source :
-
Animal Reproduction Science . Jan2013, Vol. 136 Issue 4, p289-295. 7p. - Publication Year :
- 2013
-
Abstract
- Abstract: The camel seminal plasma contains a diverse array of components including lipids, carbohydrates, peptides, ions and proteins. These are essential for maintaining normal physiology of spermatozoa and are secreted mainly from the prostrate, epidydimis and bulbo-urethral glands of reproductive system. The protein profiles of camel seminal plasma were resolved by two-dimensional gel electrophoresis (2D-PAGE). The majority of the protein was found in acidic regions below pI 7.0 and the 19 brightly stained proteins were identified by MALDI-TOF/MS analysis. On the basis of proteomic profiles, β-nerve growth factor (β-NGF) was purified by ion-exchange and gel filtration chromatography and identified by SDS-PAGE and MALDI-TOF/MS analysis. It was further confirmed by western blotting experiments using rabbit anti-β-NGF primary antibody. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 03784320
- Volume :
- 136
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Animal Reproduction Science
- Publication Type :
- Academic Journal
- Accession number :
- 84574143
- Full Text :
- https://doi.org/10.1016/j.anireprosci.2012.11.001