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Estimation of sertraline by chromatographic (HPLC-UV273 nm) technique under hydrolytic stress conditions.
- Source :
-
Pharmaceutical Methods . Jul-Dec2012, Vol. 3 Issue 2, p62-67. 6p. - Publication Year :
- 2012
-
Abstract
- Purpose: In this paper, simple, specific and accurate RP-HPLC method was developed in order to study decomposition of sertraline (SRT) under the hydrolytic stress conditions (acid, neutral, alkaline and oxidative). Materials and Methods: The best separation of SRT and its degradation products were achieved on reverse phase LiChoCART with Purospher (RP-18e) column. The mobile phase was composed of methanol/water (75:25, v/v). The detection wavelength was 273 nm. The method was validated and response was found to be linear in the drug concentration range of 10-200 µg ml-1 with correlation coefficient of 0.998. Results: The RSD values for intra- and inter-day precision were < 0.65 and < 0.72%, respectively. Employing RP-HPLC method, degradation products were detected in the exposed samples. Conclusion: It was found that the susceptibility of SRT to hydrolytic decomposition increased in following manner: Neutral condition < alkaline condition < acid condition < oxidative condition. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 22294708
- Volume :
- 3
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Pharmaceutical Methods
- Publication Type :
- Academic Journal
- Accession number :
- 84534120
- Full Text :
- https://doi.org/10.4103/2229-4708.103874