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A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice.
- Source :
-
Journal of Integrative Plant Biology . Dec2012, Vol. 54 Issue 12, p979-990. 12p. - Publication Year :
- 2012
-
Abstract
- A thorough understanding of the genetic basis of rice grain traits is critical for the improvement of rice ( Oryza sativa L.) varieties. In this study, we generated an F2 population by crossing the large-grain japonica cultivar CW23 with Peiai 64 (PA64), an elite indica small-grain cultivar. Using QTL analysis, 17 QTLs for five grain traits were detected on four different chromosomes. Eight of the QTLs were newly-identified in this study. In particular, qGL3-1, a newly-identified grain length QTL with the highest LOD value and largest phenotypic variation, was fine-mapped to the 17 kb region of chromosome 3. A serine/threonine protein phosphatase gene encoding a repeat domain containing two Kelch motifs was identified as the unique candidate gene corresponding to this QTL. A comparison of PA64 and CW23 sequences revealed a single nucleotide substitution (C→A) at position 1092 in exon 10, resulting in replacement of Asp (D) in PA64 with Glu (E) in CW23 for the 364th amino acid. This variation is located at the D position of the conserved sequence motif AVLDT of the Kelch repeat. Genetic analysis of a near-isogenic line (NIL) for qGL3-1 revealed that the allele qGL3-1 from CW23 has an additive or partly dominant effect, and is suitable for use in molecular marker-assisted selection. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 16729072
- Volume :
- 54
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Journal of Integrative Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 84187108
- Full Text :
- https://doi.org/10.1111/jipb.12008