Back to Search
Start Over
Enhancement of n-decane hydroxylation activity of Monilliera sp. NAP 00702 in a liquid–liquid interface bioreactor by mixing of anion-exchange resin microparticles
- Source :
-
Process Biochemistry . Dec2012, Vol. 47 Issue 12, p2494-2499. 6p. - Publication Year :
- 2012
-
Abstract
- Abstract: Fungal cells immobilized with ballooned polyacrylonitrile (PAN) microsphere (MS) on an interface between a liquid medium and a hydrophobic organic solvent efficiently catalyze various microbial transformations such as hydrolysis, reduction, hydroxylation, and epoxidation of lipophilic substrates dissolved in the organic phase (liquid–liquid interface bioreactor; L–L IBR). In this study, the electric charge of MS layer in the L–L IBR was modified by mixing 4 kinds of anion-exchange, 2 kinds of cation-exchange or a chelating resin microparticles, and applied to regio- and stereoselective subterminal hydroxylation of n-decane to (−)-4-decanol by Monilliera sp. NAP 00702. In general, the addition of cation-exchange and chelating resin microparticles into a PAN-MS layer led to the strong inhibition of fungal growth and hydroxylation activity. On the other hand, the addition of anion-exchange resin microparticles having moderate total capacity (≤1.00meq/g) significantly increased hyphal growth and hydroxylation activity. Especially, the addition of IRA910CT microparticles (total capacity≥1.00meq/g) into a PAN-MS layer led to 1.6-fold increase of 4-decanol production. Thus, it was concluded that the moderate addition of anion-exchange resin microparticles into an MS layer in the L–L IBR system was effective to enhancement of fungal growth and hydroxylation activity. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 13595113
- Volume :
- 47
- Issue :
- 12
- Database :
- Academic Search Index
- Journal :
- Process Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 84162494
- Full Text :
- https://doi.org/10.1016/j.procbio.2012.10.013