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Hydrogen/deuterium exchange reflects binding of human centrin 2 to Ca2+ and Xeroderma pigmentosum Group C peptide: An example of EX1 kinetics

Authors :
Sperry, Justin B.
Ryan, Zachary C.
Kumar, Rajiv
Gross, Michael L.
Source :
International Journal of Mass Spectrometry. Dec2012, Vol. 330-332, p302-309. 8p.
Publication Year :
2012

Abstract

Abstract: Xeroderma pigmentosum (XP) is a genetic disease affecting 1 in 10,000–100,000 and predisposes people to early-age skin cancer, a disease that is increasing. Those with XP have decreased ability to repair UV-induced DNA damage, leading to increased susceptibility of cancerous non-melanomas and melanomas. A vital, heterotrimeric protein complex is linked to the nucleotide excision repair pathway for the damaged DNA. The complex consists of XPC protein, human centrin 2, and RAD23B. One of the members, human centrin 2, is a ubiquitous, acidic, Ca2+-binding protein belonging to the calmodulin superfamily. The XPC protein contains a sequence motif specific for binding to human centrin 2. We report here the Ca2+-binding properties of human centrin 2 and its interaction with the XPC peptide motif. We utilized a region-specific H/D exchange protocol to localize the interaction of the XPC peptide with the C-terminal domain of centrin, the binding of which is different than that of calmodulin complexes. The binding dynamics of human centrin 2 to the XPC peptide in the absence and presence of Ca2+ are revealed by the observation of EX1 H/D exchange regime, indicating that a locally unfolded population exists in solution and undergoes fast H/D exchange. [Copyright &y& Elsevier]

Details

Language :
English
ISSN :
13873806
Volume :
330-332
Database :
Academic Search Index
Journal :
International Journal of Mass Spectrometry
Publication Type :
Academic Journal
Accession number :
83934945
Full Text :
https://doi.org/10.1016/j.ijms.2012.10.013