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Phase-sensitive surface plasmon resonance biosensors: methodology, instrumentation and applications.
- Source :
-
Annalen der Physik . Nov2012, Vol. 524 Issue 11, p637-662. 26p. - Publication Year :
- 2012
-
Abstract
- Surface Plasmon Resonance (SPR) has become a central tool for label-free characterization of biomolecular interactions. Based on monitoring of amplitude characteristics, conventional SPR sensors have been extensively explored, commercialized and applied for studies of many important interactions (antigen-antibody, protein-ligand etc), but this technology still lacks of sensitivity for the detection of relatively small and low copy number compounds. Phase-sensitive SPR has recently emerged as an upgrade of this technology to resolve the sensitivity issue. Profiting from a sharp phase jump under SPR and ultra-sensitive tools of its control, this technology offers up to 100-time improvement of the detection limit, giving access to the detection of trace amounts of small molecular weight analytes (drugs etc). This paper intends to provide a tutorial on basic concepts of phase detection in SPR sensing, compare the performance of phase- and amplitude-sensitive sensors, review recent progress in the development and applications of phase-sensitive SPR sensors, and outline future prospects and trends of this technology. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00033804
- Volume :
- 524
- Issue :
- 11
- Database :
- Academic Search Index
- Journal :
- Annalen der Physik
- Publication Type :
- Academic Journal
- Accession number :
- 83487346
- Full Text :
- https://doi.org/10.1002/andp.201200203