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Essential oil of tarragon (Artemisia dracunculus) antibacterial activity on Staphylococcus aureus and Escherichia coli in culture media and Iranian white cheese.
- Source :
-
Iranian Journal of Microbiology . Mar2012, Vol. 4 Issue 1, p30-33. 4p. - Publication Year :
- 2012
-
Abstract
- Background and Objectives: In this study, the antibacterial effect of essential oil of tarragon (Artemisia dracunculus) on Staphylococcus aureus and Escherichia coli was evaluated in culture media and Iranian white cheese. Materials and Methods: The tarragon essential oil (EO) obtained by the steam distillation method and its antibacterial activity was evaluated in 96-well microtiter plates containing brain heart infusion broth. The enumeration of S. aureus and E. coli in cheese samples were carried out on the following media: Baired parker agar for S.aureus, incubated at 37 °C for 24 h; and MacConkey sorbitol agar for E. coli, incubated at 37°C for 24 h. Iranian white cheese was produced from fresh and whole pasteurized cow milk (2.5%). Bacteria (103 cfu/mL) were inoculated to different batches. Cheese was treated with different concentrations of EO (15 and 1500 µg/mL) and separated into four parts in an equal manner. The sensory evaluation was done by a panel of four judges. Results: According to the results obtained, minimum inhibitory concentrations (MIC) for E. coli and S. aureus were 2500 and 1250 µg/mL, respectively. Also, minimum bactericidal concentration (MBC) for the mentioned microorganisms were 5000 and 2500 µg/mL, respectively. All the EO concentrations for each bacteria result in reducing bacterial count of cheese samples compared to control (P < 0.05). Also, with increasing concentration of EO in cheese samples, the bacterial count was reduced further (P < 0.05). Conclusion: Based on our findings, tarragon essential oil has antibacterial effect on two important pathogen bacteria (S. aureus and E. coli) and can be applied as a preservative in foods such as cheese. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20083289
- Volume :
- 4
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Iranian Journal of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 83457420