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Silver, gold, and alloyed silver-gold nanoparticles: characterization and comparative cell-biologic action.
- Source :
-
Journal of Nanoparticle Research . Oct2012, Vol. 14 Issue 10, p1-13. 13p. 2 Color Photographs, 4 Black and White Photographs, 2 Charts, 4 Graphs. - Publication Year :
- 2012
-
Abstract
- Silver, gold, and silver-gold-alloy nanoparticles were prepared by citrate reduction modified by the addition of tannin during the synthesis, leading to a reduction in particle size by a factor of three. Nanoparticles can be prepared by this easy water-based synthesis and subsequently functionalized by the addition of either tris(3-sulfonatophenyl)phosphine or poly( N-vinylpyrrolidone). The resulting nanoparticles of silver (diameter 15-25 nm), gold (5-6 nm), and silver-gold (50:50; 10-12 nm) were easily dispersable in water and also in cell culture media (RPMI + 10 % fetal calf serum), as shown by nanoparticle tracking analysis and differential centrifugal sedimentation. High-resolution transmission electron microscopy showed a polycrystalline nature of all nanoparticles. EDX on single silver-gold nanoparticles indicated that the concentration of gold is higher inside a nanoparticle. The biologic action of the nanoparticles toward human mesenchymal stem cells (hMSC) was different: Silver nanoparticles showed a significant concentration-dependent influence on the viability of hMSC. Gold nanoparticles showed only a small effect on the viability of hMSC after 7 days. Surprisingly, silver-gold nanoparticles had no significant influence on the viability of hMSC despite the silver content. Silver nanoparticles and silver-gold nanoparticles in the concentration range of 5-20 μg mL induced the activation of hMSC as indicated by the release of IL-8. In contrast, gold nanoparticles led to a reduction of the release of IL-6 and IL-8. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13880764
- Volume :
- 14
- Issue :
- 10
- Database :
- Academic Search Index
- Journal :
- Journal of Nanoparticle Research
- Publication Type :
- Academic Journal
- Accession number :
- 82536537
- Full Text :
- https://doi.org/10.1007/s11051-012-1153-5