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Evidence of IgY Subclass Diversification in Snakes: Evolutionary Implications.
- Source :
-
Journal of Immunology . 10/1/2012, Vol. 189 Issue 7, p3557-3565. 9p. - Publication Year :
- 2012
-
Abstract
- Mammalian IgG and IgE are thought to have evolved from IgY of nonmammalian tetrapods; however, no diversification of IgY sub-classes has been reported in reptiles or birds, which are phylogenetically close to mammals. To our knowledge, we report the first evidence of the presence of multiple IgY-encoding (v) genes in snakes. Two v genes were identified in the snake Elaphe taeniura, and three v genes were identified in the Burmese python (Python molurus bivittatus). Although four of the v genes displayed a conven-tional four-H chain C region exon structure, one of the v genes in the Burmese python lacked the H chain C region 2 exon, thus exhibiting a structure similar to that of the mammalian 7 genes. We developed mouse mAbs specific for the IgYl and IgY2 of E. taeniura and showed that both were expressed in serum; each had two isoforms: one full-length and one truncated at the C terminus. The truncation was not caused by alternative splicing or transcriptional termination. We also identified the (jl and 8 genes, but no a gene, in both snakes. This study provides valuable clues for our understanding of Ig gene evolution in tetrapods. [ABSTRACT FROM AUTHOR]
- Subjects :
- *IMMUNOGLOBULINS
*SNAKE ecology
*TETRAPODS
*PHYLOGENY
*SERUM
*GENE expression
Subjects
Details
- Language :
- English
- ISSN :
- 00221767
- Volume :
- 189
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- Journal of Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 82183049
- Full Text :
- https://doi.org/10.4049/jimmunol.1200212