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Detection of urine cofilin-1 from patients hospitalized in the intensive care unit using the metal-enhanced fluorescence technique
- Source :
-
Sensors & Actuators B: Chemical . Oct2012, Vol. 173, p184-190. 7p. - Publication Year :
- 2012
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Abstract
- Abstract: Ischemic shock and acute kidney injury (AKI) are the primary causes of high mortality in the intensive care unit (ICU) patients. In a rat model, ischemia is able to disrupt the actin cytoskeleton of proximal tubule cells. This effect is associated with the expression and excretion of actin depolymerizing factor (ADF)/cofilin. However, the human evidence of ADF/cofilin excreted in ICU patients with ischemic shock and/or AKI remains to be addressed. Here we developed a 96-well high-throughput, localized surface plasmon-coupled fluorescence biosensor (HT-LSPCFB) combining a sandwich immunoassay to measure the urine cofilin-1 in 57 ICU patients and 8 healthy controls. A linear relationship between 1 and 105 pg/mL of human cofilin-1 recombinant protein was determined (R 2 =0.9845) in this study. The highest normalized cofilin-1 levels obtained in the ICU patients and healthy adults were 1.985 and 0.726, respectively. The mean normalized cofilin-1 level of total ICU patients (0.7403) was also significantly higher than that of healthy adults (0.4759) (p =0.0052). An examination of the receiver operating characteristic (ROC) curve and area under curve (AUC) showed that cofilin-1 is acceptable for assessing the ICU patients (AUC=0.775) and shock (AUC=0.713), but not for AKI (AUC=0.681). Therefore, the HT-LSPCFB is suitable for detecting the urine cofilin-1 which potentially becomes a prognostic factor for ICU patients with shock. [Copyright &y& Elsevier]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 173
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 82106788
- Full Text :
- https://doi.org/10.1016/j.snb.2012.06.076