Prolifération et signatures ADN de complexité génomique pour la définition du pronostic des carcinomes mammaires.

Invasive breast carcinoma's prognosis is defined using clinico-pathological parameters among which proliferation is essential. Ki67 has strong prognostic and predictive values. Ki67 is expressed during all the cell cycle but G0. The determination of its expression with the MIB1 antibody, on tissue sections, requires well-controlled preanalytical steps for tissue preparation (fixation and paraffinembedding) and an accurate protocol for immunohistochemistry. These technical issues are limiting but largely balanced with immunohistochemistry's low cost and its wide utilization in pathology laboratories. Ki67 staining's interpretation follows international guidelines recently published without any recommended cut-off. However, the most commonly used is 20% of stained cells. Genome complexity assessed with DNA arrays provides another level of prognostic information. The higher the number of chromosome breakpoints, the poorer the prognosis. However, until now, this tool remains useful in the context of clinical trials in specialized centers. [ABSTRACT FROM AUTHOR]