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The second transcribed spacer rDNA sequence: an effective genetic marker for inter-species phylogenetic analysis of trematodes in the order Strigeata.
- Source :
-
Parasitology Research . Oct2012, Vol. 111 Issue 4, p1467-1472. 6p. - Publication Year :
- 2012
-
Abstract
- In the present study, the second nuclear internal transcribed spacer (ITS-2) rDNA of Schistosoma japonicum isolates in mainland China was amplified, sequenced, and assessed for inferring the intra- and inter-species phylogenetic relationships of trematodes in the order Strigeata. The fragment containing ITS-2 rDNA was obtained from 24 S. japonicum isolates from eight epidemic provinces in mainland China. The length polymorphisms were observed among these ITS-2 rDNA sequences, ranging from 343 to 346 bp, and the intra- and inter-population variations in ITS-2 sequence were 0.0-2.1 % among S. japonicum isolates in China. Phylogenetic analyses using the maximum parsimony and maximum likelihood methods revealed that the ITS-2 rDNA sequence is not a suitable marker for studying inter- and intra-population variation in S. japonicum. However, phylogenetic analysis of trematodes in the order Strigeata indicated that the ITS-2 rDNA sequence provides an effective molecular marker for studying inter-species phylogenetic relationships among trematodes in this order. [ABSTRACT FROM AUTHOR]
- Subjects :
- *RECOMBINANT DNA
*GENETIC transcription
*BIOMARKERS
*SCHISTOSOMA japonicum
Subjects
Details
- Language :
- English
- ISSN :
- 09320113
- Volume :
- 111
- Issue :
- 4
- Database :
- Academic Search Index
- Journal :
- Parasitology Research
- Publication Type :
- Academic Journal
- Accession number :
- 80125825
- Full Text :
- https://doi.org/10.1007/s00436-012-2981-z