Improvements in the enzymatic synthesis of phosphatidylserine employing ionic liquids

Abstract: The aim of this work was to investigate innovative reaction media such as ionic liquids for enzymatic phospholipids transformations in order to get a higher selectivity in the enzymatic transphosphatidylation reactions of natural phosphatidylcholine catalyzed by phospholipase D (PLD) from Streptomyces PMF. The stability and the activity of the PLD in the ionic liquid [BMIM][PF6] were tested. This ionic liquid used as a cosolvent in the synthesis of phosphatidylserine lead surprisingly to the almost quantitative suppression of unwanted hydrolytic side reaction, thus providing a great improvement in the final product purification. [Copyright &y& Elsevier]