Antioxidant Activity of Durian (Durio zibethinus Murr.) Shell In vitro.

Objective: The paper tried to systematically investigate the antioxidant potential of Durian (Durio zibethinus Murr.) shell in vitro then to provide the evidence for its further development in future. Methods: The antioxidant activity of methanol extract of durian shell (MEDS) was evaluated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH ) radical, 2,2'-azino-bis (3- ethylbenzthiazoline-6-sulfonic acid) (ABTS+ ) radical, reducing power (Fe3+ & Cu2+), superoxide anion radical (O.-2 ), hydroxyl radical (.OH), anti-lipid peroxidation and ferrous ions (Fe2+) chelating activity assays, compared with positive control Trolox. The total phenol contents were also determined by Folin-Ciocalteu reagent. Results: MEDS exhibited effective antioxidant abilities in dose-dependent manners, its IC values were calculated as 102.37±1.98, 19.50±1.44, 280.79±22.80, 50 154.67±5.84, 770.52±19.28, 324.63±17.61, 4.45±1.13 and 63.95±14.91 μg/mL, respectively, for DPPH, ABTS+, reducing power (Fe3+), reducing power (Cu2+), superoxide anion scavenging, hydroxyl scavenging, anti-lipid peroxidation, chelating ability (Fe2+) assays. The total phenol contents (33.77±1.77 mg GAE/g) were found in MEDS. Conclusion: Durian (Durio zibethinus Murr.) shell possesses effective in vitro antioxidant ability which may contribute to its pharmacological effects or healthcare functions. It exerted the antioxidant possibly via metal-chelating and radicalscavenging which may result from donating hydrogen atom (H.) and electron (e). Its antioxidant ability can be attributed to the total phenol. As a waste generally discarded, durian shell can be a source of useful natural antioxidant for medical or health care. [ABSTRACT FROM AUTHOR]