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Expression of Atropa belladonna Putrescine N-Methyltransferase Gene in Root Pericycle.
- Source :
-
Plant & Cell Physiology . Mar1999, Vol. 40 Issue 3, p289-297. 9p. - Publication Year :
- 1999
-
Abstract
- The cDNAs encoding putrescine N-methyltransferase (PMT), which catalyzes the S-adenosylmethionine-dependent N-methylation of putrescine at the first committed step in the biosynthetic pathways of tropane alkaloids, were isolated from Atropa belladonna and Hyoscyamus niger. These PMTs, however, lacked the N-terminal tandem repeat arrays previously found in Nicotiana PMTs. AbPMT1 RNA was much more abundant in the root of A. belladonna than was AbPMT2 RNA. The 5′-flanking region of the AbPMT1 gene was fused to the β-glucuronidase (GUS) reporter gene and transferred to A. belladonna. Histochemical analysis showed that GUS is expressed specifically in root pericycle cells and that the 0.3-kb 5′-upstream region was sufficient for pericycle-specific expression. Treatment of A. belladonna roots with methyl jasmonate did not up-regulate the expression of GUS or endogenous AbPMT genes. The regulation of tropane alkaloid biosynthesis is discussed and compared with that of nicotine biosynthesis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00320781
- Volume :
- 40
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Plant & Cell Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 79307354
- Full Text :
- https://doi.org/10.1093/oxfordjournals.pcp.a029540