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Metabolic responses induced by thrombin in human umbilical vein endothelial cells
- Source :
-
Biochemical & Biophysical Research Communications . May2002, Vol. 293 Issue 3, p979. 7p. - Publication Year :
- 2002
-
Abstract
- Metabolic responses induced by thrombin in human umbilical vein endothelial cells (HUVECs) were investigated by using the cytosensor technique. Thrombin increased the extracellular acidification rate of endothelial cells, measured as an index of metabolic activity with a cytosensor microphysiometer, in a concentration-dependent fashion with an <f>EC50</f> of <f>1.27±0.59</f> IU/ml, which was abolished by the MAP kinase inhibitor PD98059. When intracellular <f>Ca2+</f> was chelated or PKC was inactivated, PD98059 failed to abolish the thrombin-induced acidification rate response in HUVECs. In addition, the tyrosine kinase inhibitor genistein, PKC inhibitor calphostin C, and <f>Na+/H+</f>exchanger antagonist MIA also partly inhibited thrombin-induced acidification rate responses. It is suggested that thrombin stimulated rapid metabolic responses via MAP kinase in HUVECs, which are calcium- and PKC-dependent. [Copyright &y& Elsevier]
- Subjects :
- *THROMBIN
*ACIDIFICATION
*ENDOTHELIUM
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 293
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 7925483
- Full Text :
- https://doi.org/10.1016/S0006-291X(02)00339-X