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5T4 Interacts with TIP-2/GIPC, a PDZ Protein, with Implications for Metastasis
- Source :
-
Biochemical & Biophysical Research Communications . Jan2002, Vol. 290 Issue 3, p1030. 7p. - Publication Year :
- 2002
-
Abstract
- Overexpression of the 5T4 transmembrane glycoprotein can have marked effects on both the actin cytoskeleton and cell migration. Using a yeast two-hybrid approach, we describe a novel interaction between 5T4 and TIP-2/GIPC, a cytoplasmic interacting protein containing a PDZ domain. The cytoplasmic tail of 5T4 contains a class I PDZ-binding motif (Ser-Asp-Val) and we demonstrate that this region, in particular the terminal valine, is required for 5T4 interaction with TIP-2/GIPC. HeLa cells expressing hemagglutinin-tagged TIP-2/GIPC (HA-TIP-2/GIPC) have an altered distribution of endogenous 5T4, which colocalizes with HA-TIP-2/GIPC, thus supporting an interaction. Furthermore, TIP-2/GIPC can be coimmunoprecipitated with 5T4 from HeLa cell lysates. Identification of the 5T4 and TIP-2/GIPC interaction provides the first link between 5T4 and the actin cytoskeleton. Since other proteins, like 5T4, associate with TIP-2/GIPC and are linked with cancer, we explore the possibility that TIP-2/GIPC may be a common factor involved in the cancer process. [Copyright &y& Elsevier]
- Subjects :
- *GLYCOPROTEINS
*CELL migration
*ACTIN
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 290
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 7924416
- Full Text :
- https://doi.org/10.1006/bbrc.2001.6288