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Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) in the cerebellum and precerebellar nuclei of the rat
- Source :
-
Molecular Brain Research . Oct2002, Vol. 107 Issue 1, p23. 9p. - Publication Year :
- 2002
-
Abstract
- Expression of inorganic phosphate/vesicular glutamate transporters (BNPI/VGLUT1 and DNPI/VGLUT2) was studied in the cerebellum and precerebellar nuclei of rats using immunohistochemistry and in situ hybridization. DNPI/VGLUT2-stained mossy fibers were principally seen in the vermis (lobules I and VIII–X) and flocculus, whereas BNPI/VGLUT1-stained mossy fibers were localized throughout the cortex. Some vermal and floccular mossy fibers were stained for both transporters. High levels of DNPI/VGLUT2 mRNA hybridization signals were demonstrated in many neurons throughout the vestibular nuclear complex as well as the lateral reticular, external cuneate, inferior olivary and deep cerebellar nuclei. Significant BNPI/VGLUT1 mRNA signals were demonstrated in the lateral reticular nucleus and vestibular nuclear complex but not in the inferior olivary nucleus, indicating that climbing fibers have DNPI/VGLUT2 only. These results show that DNPI/VGLUT2 is expressed preferentially to vestibulo-, reticulo- and cuneocerebellar neurons, some of which also possess BNPI/VGLUT1, suggesting some differential and co-operative functions between DNPI/VGLUT2 and BNPI/VGLUT1 in the cerebellum. [Copyright &y& Elsevier]
- Subjects :
- *IMMUNOHISTOCHEMISTRY
*IN situ hybridization
*CEREBELLUM
Subjects
Details
- Language :
- English
- ISSN :
- 0169328X
- Volume :
- 107
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Molecular Brain Research
- Publication Type :
- Academic Journal
- Accession number :
- 7912424
- Full Text :
- https://doi.org/10.1016/S0169-328X(02)00442-4